Composite

Part:BBa_K4015007:Design

Designed by: Andrew Peng Yi   Group: iGEM21_KEYSTONE   (2021-10-19)


CotA-Lcp-HlyA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1348
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 286


Design Notes

The cotA should be directly bind to hlyA because this hinder both of their function.


Source

The parts are assembled by Gibson assembly, and the product is ligated into pET 23a.

References